Classified Title: Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $37,781 – $51,950 Employee group: Full Time Schedule: M-F 8:30 -5:00 Employee subgroup: Salaried Exmpt Location: 855 N. Wolfe Street Department name: 10003050-SOM Pat Gastrointestinal Liver Pathology Personnel area: School of Medicine
Advanced research and/or clinical laboratory specialist with a firm understanding of techniques and a solid foundation in the basic biology of gastrointestinal epithelium, gained from some professional experience.
Performs clinical and research laboratory experiments by following established procedures and protocols. Participates in the design of experiments and analyzes data. Works independently and manages daily research projects. The Research Specialist II will assist the principal investigator in the development and coordination of projects, experimentation, data collection/maintenance, and organization of the laboratory. Identifies and resolves problems and uses analytical and decision making skills to implement assigned projects. Collects and documents for presentations, manuscripts, grants, and reporting. Serves as primary lab contact in the absence of Principal Investigator.
Duties & Responsilities
Performs clinical and research lab tests and experiments. Organizes workflow and ensures laboratory meets all regulatory or research requirements. Follows established procedures and protocols, utilizing appropriate technique to develop, perform and analyze laboratory tests and/or experiments which may include 3D intestinal organoid culture, molecular cloning, genome editing, FACS analysis, western blotting, PCR, tissue microarray construction, immunohistochemistry, immunofluorescence, confocal/advanced microscopy, and mouse work
Reports and records outcomes/results of all tests. Assists in the collection and documentation of data for reports, presentations, manuscripts/publications, and grant documentation. Exchanges routine information in an appropriate manner requiring good oral and written communication. Regular interaction with students, vendors, post-doctoral fellows, administrative assistants, and other faculty members.
Serves as primary laboratory contact in the absence of PI/Faculty. May be responsible for routine operational activities such as; ordering supplies and materials, reviewing inventory, maintaining up to date records of tests, etc. Preventative maintenance of equipment. Reconciling budgets, laboratory purchases, etc.
Equipment, Machine, or Tool Requirements
Computer and laboratory equipment.
Operates lab equipment such as: tissue culture hood, pipettes, incubators, water baths, heat blocks, shakers, thermocyclers, autoclave, compressed gas canisters, glass containers, and microscopes.
Bachelor's degree in biological sciences, chemistry, or related field required.
Master's or other advanced degree preferred.
Minimum of 2 years related clinical and/or research laboratory experience performing relevant or related techniques required. Knowledge of good lab practices required. Individual must be able to work independently, under minimal supervision, with strong decision making skills, effective communication, and good judgment. Ability to critically review and synthesize knowledge/ideas from scientific literature is required.
Special Knowledge, Skills, or Abilities / Competencies
Experience with 3D intestinal organoid culture, or at least mammalian tissue culture, is strongly preferred. Independence, creativity, enthusiasm, and a collaborative attitude are required.
Work Environment /While performing the duties of this job
While performing the duties of this job, the employee may remain in a stationary position (sitting or standing) up to 60% of the time. Occasionally required to traverse throughout the laboratory or to retrieve equipment, supplies, or specimens from other locations on campus. The employee is required to operate computer keyboard, laboratory instruments, and small laboratory tools such as pipettes, cutting instruments, glass tubes, dishes, and/or slides. Constantly repositions self to perform laboratory experiments.
Prolonged vision requirements including viewing computer screen, lab notebook, and scientific papers. Visual acuity is required to distinguish fine gradation of color or structure and closely examine specimens (including via a microscope).
Laboratory environment - Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed. Must refer to and expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas. Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area. Working in laboratory where there may be discomforts due to odors, noise, temperature fluctuations, and working around lab equipment. Research areas may include animal, human, human products, DNA, and radiation protocols. Use of personal protective equipment may be advised or required.
May transport reagents and supplies usually less than 5 pounds from one area to another (in the laboratory or on campus; to transport between laboratories or to bring materials to meetings).
Staff may be required to complete annual competency review, and must insure compliance with Johns Hopkins Policy, Laboratory Policy & Procedure, Health, Safety & Environment regulations, and all applicable privacy & confidentiality laws/practices.
Report to site may be required in weather or declared emergencies if participating in animal work.
The successful candidate(s) for this position will be subject to a pre-employment background check.
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The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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