UCSD Layoff from Career Appointment: Apply by 5/15/18 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor. Eligible Disability Counseling and Consulting services (DCC) or Special Selection clients should contact their Vocational Rehabilitation Counselor for assistance.
The UCSD Stem Cell Program, Sanford Stem Cell Clinical Center, and the Sanford Consortium for Regenerative Medicine are three of the University's most highly visible and top priority interdisciplinary and multi-institutional programs. Program personnel work on a daily basis with various academic and administrative Vice Chancellors due to the complexity of these programs and substantial institutional investment. In addition, these programs are highly covered by local and international newspapers and other media outlets, including ethically sensitive stem cell research and cutting edge therapy development efforts. These advancements involve substantial investments of UCSD financial physical, and human resources.
The Human Embryonic Stem Cell Core Facility (hESCCF) is a medium-sized laboratory in the UCSD Stem Cell Program providing services to an increasing number of research users who pay recharge costs to the facility. The current number of frequent users is 30. Created in 2005, the hESCCF is a cornerstone of the UCSD Stem Cell Program and the UC San Diego institutional commitment to leadership in Regenerative Medicine. It fosters and promotes human Embryonic Stem Cell research at UC San Diego and partner institutions of the Sanford Consortium for Regenerative Medicine. The facility trains scientists in the basic methods to conduct human Embryonic Stem Cell research, facilitates hESC research for many investigators, both established and beginning scientists, provides a "safe haven" that is sheltered from any federal funding source, thus allowing unimpeded hESC research, and provides state-of-the-art technologies necessary to advance stem cell research. hESCCF sustains ongoing research projects and allows the proposal of new projects necessitating such facilities.
Under supervision, the Stem Cell Research Associate will assist in the operation and maintenance of three Fluorescence Activated Cell Sorters (FACS) and three bench top flow cytometers. Perform standard repetitive laboratory procedures with Human Embryonic Stem Cells and other established cell lines and primary tissues. Conduct a limited number of experimental procedures and achieve results to maintain and expand hES cell lines and other pluripotent stem cells. Assist with the evaluation of new reagents and protocols. Support the preparation of detailed analysis of results for experiments such as qPCR, fluorescent and confocal microscopy, gel imaging, and proliferation assays. Under close technical supervision, assist with protocol troubleshooting to help researchers overcome technology bottlenecks. Receive training, such as technical seminars, to learn how to integrate emerging technologies into the core facility.
Theoretical knowledge of molecular biology, cellular biology or related field as typically attained through a bachelor's degree or an equivalent combination of education and experience.
Demonstrated ability to perform repetitive and specialized laboratory experimental procedures. Ability to follow experimental protocols. Ability to learn new procedures.
Experience and working knowledge of sterile techniques and mammalian cell culture practices. Knowledge of numerous types of laboratory equipment related to uses, maintenance, and repair.
Acute attention to detail. Demonstrated ability to be resourceful and capable of handling the changing needs of the research lab.
Excellent multi-tasking and organization skills to conduct a variety of tasks in an extremely fast-paced environment.
Exceptional communication skill in written and spoken form. Demonstrated ability to communicate effectively in writing, electronically, and by spoken word with a variety of people from vendors to faculty.
Theoretical or practical knowledge of human or murine stem cell culture.
Working practical knowledge of Flow Cytometry technology.
Experience using microscopy to perform experiments.
Employment is subject to a criminal background check.
Must be able to work in environment where biological, chemical, and radiological hazards are present, and human embryonic stem cell and animals are used in research.
Occasional overtime, including nights, weekends and holidays may be required.
Must be willing to work with viruses that are infe
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